Film Analysis :: essays research papers

Film Assignment I have this opinion that America â€Å"a land of opportunity† and also a â€Å"rat race.† Why I say both of them? First, it is because, for me, America is a land of opportunity. Everybody has the opportunity to work as long as they have the willing to work, the work ethic. It’s just not everybody has the willing to work because the government will support those people who are unemployed. Like foreign students for example. The INS gives foreign students the â€Å"practical training† permit, so they can use it for working in the US after they finish their school. Even though it’s only a one-year permit, at least they have the opportunity to look for a company who is willing to be their sponsor for applying for the H-1 visa. Like in the movie It’s a Wonderful Life. When George Bailey got bankrupt, he has another chance to make everything back to normal if he wanted to. However, when we look at the â€Å"individual social mobility, it makes Amer ica a â€Å"rat race† country. Since America is known as a land of opportunity, which will lead into a â€Å"rat race† because people may want to accomplish or fulfil their goal. Everybody is trying to get the highest position in his or her job. Everybody is trying to make higher profit in his or her life. They will do anything to get into that â€Å"position† even though they have to do something illegal, something bad, something deceitful, corruption. Everybody is competing and just thinks about him or her self. Like in the Glengarry Glen Ross, everybody is trying to keep his job there. They have to make the highest profit or they will lose their job, and that makes some of the officers rob the office. It’s a beautiful life is a kind of family movie that has a happy ending. When I watched it, I got so depressed in the middle of the movie because everything happens is just like a tragedy. That is the basic story after all. George Bailey has been dealt a savage blow and is contemplating suicide at his lowest ebb. It takes an apprentice angel to put it all in perspective and remind him that joy in life should never be eclipsed by any setback. After all how many of us can empathize with a penny pinching self centered old grouch versus a good man faced with letting his family and friends down.

Lord of The Rings/Kite Runner Compare and Contrast Essay

What objects do you associate innocence with? Marriage, virginity, a childhood toy? When we think of dominance we think of war; we think of negativity. When the phrase ‘parental influence’ comes to mind, we go to our mothers tucking us into bed and watching the game with our fathers—at least that is what us lucky ones think of. Not everyone is lucky enough to have that innocence stored forever, violent free lives, and a mother and father by our side.The Kite Runner and Lord of the Flies have many similarities, particularly when comparing themes such as loss of innocence, power and dominance, and paternal influences and the ways in which they are depicted through symbolism and irony. From the beginning of The Kite Runner Hoseinni showed innocence through Amir’s passion and longing for kites, but not any kite; Amir longed for the winning kite in the annual kite flying tournament. Kites were constantly present during Amir’s childhood. They were his good ness and his purity; the goodness and purity of Afghanistan at that time.Amir’s innocence was stolen by him when he was twelve-years-old by the neighborhood bully, Assef. Amir longed for the winning kite, but at the expense of his friend, Hassan: â€Å"But there were two things amid the garbage that I couldn’t stop looking at: One was the blue kite resting against the wall,† (75). Amir witnessed the rape of Hassan, and after he took his trophy kite home and hung it up on the wall it mocked him, reminding him of his cowardice, and the purity and the innocence that were taken from him. When Amir returned to Kabul as an adult there were no kites.Afghanistan had been run over by the Taliban and everything had been destroyed, just like the innocence that was taken by Amir and the goodness and that was stolen from him. In The Kite Runner, Amir wanted the winning kite. After witnessing the rape of Hassan—witnessing his beloved friend sacrifice himself for the fi rst place kite and seeing Kabul in ruin with the kites gone and the tournaments ended, he craves the innocence and goodness of his past. In Lord of the Flies, Golding used symbols very similarly to Hoseinni’s use of the kite as a symbol of innocence.Instead of an object or toy, Golding used Simon to show purity. Simon, the quiet and kind hearted boy, was murdered savagely by his peers. Simon had been the good and the innocent on the island; unlike the other boys he knew that the evil was inside of the others, though he himself had been too good for the evil. Simon was taken away from the boys but not by outsiders, like the Taliban; the boys themselves took Simon away, unlike The Kite Runner where Assef and the Taliban took the innocence away from Amir. â€Å"There were no words, and no movements but the tearing of teeth and claws† (153).The boys turned into savages and took Simon away from themselves. Unlike The Kite Runner, Simon and innocence were not wanted and were easily thrown away. As goodness was being murdered, the boys turned into monsters. Not once did they think of what was being depleted. Another theme shared by the two novels is the idea of power and dominance. In The Kite Runner, Hoseinni showed this through the bully Assef, who later became a Taliban official. Not only did Hoseinni use Assef and the Taliban as symbols of power, but he enforced them through irony.One example of dramatic irony in The Kite Runner took place when Baba was talking to Amir as a child. Baba said to him, â€Å"’God help us all if Afghanistan ever falls into their hands! ’† (17). Baba was referring to the Taliban and how he hoped they would never come into power, though consequently, the Taliban later dominate Afghanistan. Baba pleads to God for help if the Taliban run over, he pleads to a God that he doesn’t necessarily believe in, and ironically, the Taliban justify all of their actions with the word of God. Likewise, Golding used Jack and his hunters to illustrate power and dominance in Lord of the Flies.In order to assert their dominance Jack and his hunters murdered the pig in an ostentatious manner thinking that being flashy and strong will bring respect. Not only did Jack allege his power through conspicuous hunting acts, but he also imposed power with his violent mannerisms towards Ralph, Piggy, and the others. Jack is not the only power figure in this story. In turn, the British official that appeared to rescue the boys has a stature of power along with the British army. Golding expressed his love of irony with the British soldier on the island as well.The boys had been creating their own war on the island, and they were, in a way, mimicking the war that had been happening on a world-wide scale. Golding did not stop here, as the British soldier then observed the boys savagery, he reprimanded them for not being more proper and British. â€Å"’I should have thought that a pack of British boy s—you’re all British, aren’t you? —would have been able to put up a better show than that—‘† (202). How ironic, that despite the soldier’s talk of being proper and â€Å"English,† he too was being a savage. He too, was in the middle of a war.Not only do The Kite Runner and Lord of the Flies share themes of innocence and power, but they both partake in the lack of positive parental influence. In The Kite Runner, Amir envied the father-son relationship that Hassan and his believed-to-be-father, Ali, shared. Like his stolen innocence, Amir yearned for his father’s approval, for these were the things he could not have. â€Å"He’d close the door, leave me to wonder why it was always grown-ups’ time with him† (5). Here, Hoseinni clearly illustrates that as growing up, and even as an adult, Amir never received the love and the affection that he so craved from his father.At every turn he would find a cl osed door; Baba would see Amir as a calamity. The one person who gave Amir positive parental influence was his father’s friend and business partner, Rahim Kahn. Amir did not take this influence to heart; his longing for approval from Baba and a mother he did not have created a hole that Rahim Kahn could not fill. With a deceased mother and apathetic father, Amir had a deep hunger for a father figure to such a degree that the lack of a parental influence caused self destruction and mental instability. In contrast to The Kite Runner, the boys in Lord of the Flies did not care for paternal influences as Amir did.In fact, they rejoiced because there were no adults on the island to keep them in check; â€Å"’Aren’t there any grownups at all? ’ ‘I don’t think so. ’ The fair boy said this solemnly; but then delight of a realized ambition overcame him’† (8). From the start, Amir wanted the parental influences that he grew up with out; the boys on the island were more than happy to be rid of them. The consequences of the lack of parental influence did not make an appearance right away, but contrary to what the boys believed, not having a parental figure did have its repercussions.One can take Roger, in consideration. At first he could not bring himself to throw stones at the smaller, younger boys. The memories of civilization and punishments still had their hold on him, but as the story progresses, Roger finds himself becoming less and less humane; much like Assef, in The Kite Runner, who as a child, subconsciously knew he could be punished for his bullying, but as he grew older and as the influence of his parents lessened, he was able to use his wrath however he pleased.The lack of parental influence in Lord of the Flies through the immature, adolescent behavior of the boys and lack of adults residing on the island leads to destruction and chaos, similar in ways to that of Amir’s, but far more externa lly dramatic. Indeed, Golding and Hoseinni share many tastes when it comes to writing, and that becomes quite apparent when one looks at the themes of the two novels. Through the loss of innocence, power and dominance, and the lack of those positive parental influences Golding and Hoseinni manage to paint the picture of a life different than the life of the average American youth.Amir kept his innocence in a kite. In his homeland. The boys on the island never knew that their innocence lay within one small boy. They didn’t have a father to look up to, a mother to run to, and neither did Amir. Now, innocence is mocked. Parental figures are pushed away, but most don’t know what it is like to lose that innocence. To not have a mother or father there in times of need. Where is your innocence stored? Where do you find the comfort and protection that are craved through a mother’s love and a father’s adoring pride?

Charles Darwin and the Theory of Evolution Essay

In the 1850s, Charles Darwin proposed his theory of evolution. His theory proposed that species evolved gradually through subtle changes from one generation to the next by means of natural selection. By natural selection, the most desirable hereditary traits become more common from one generation to the next while the less desirable, weaker traits die out. This gives rise to an organism that is more capable—fitted to—of surviving in the surrounding environment. At the time Darwin formulated his idea beginning with his trip on the HMS Beagle in the 1830s, some scientists resorted to the idea that God had preordained life by natural laws rather than by miraculous feats. While logical and correct, the idea still refers to a divine power, indicating a religious bent, so perhaps it was obvious that the ideas addressed could lead to religious controversy. As is common in science, Darwin’s concept arose from ideas garnered from a number of scientists of his time. Some researchers of the time believed that natural laws were responsible for life. While Darwin’s ideas did not account for the processes believed to account for life at the time, there was a theory at the time thought to account for life. Most thought that species were transmuted from one species into another. The problem with transmutation, an idea that is similar to evolution in some respects, is that a species may change through transmutation, but it will still be the same species. A dog may change into a different type of dog, but it will still be a dog; likewise, for a cat or any other species. Evolution dictates that the entire animal kingdom can grow through stages from one species into another over time. Birds came from reptiles, mammals came from birds and humans came from non-humans. (Lewontin, 1981) The data is unequivocal. During his voyage on HMS Beagle, Darwin found fossil remains of gigantic mammals that were recently extinct with no indication that their extinction had been caused by climate changes or catastrophic events. Although he believed that the remains he found were related to species in Africa or Europe, examination of the remains Darwin found showed that they were only related to other species found only in the Americas. Creationists insist that life came about from God in six days. While most evolutionists attack creationism on the grounds of scientific facts, there is another line of evidence virtually unnoticed by those who support evolution theory. Historians and archeologists have learned that the biblical story of creation came from the myths of another culture. Stories presented in the Bible evolved slowly over time, long before religions existed, and incorporated tales from many cultures. The story of the Garden of Eden, the serpent and the Tree of Life, for example, are said to have been depicted on an Akkadian Cylinder Seal nearly 2500 years before Christ. The serpent itself was viewed as a deity. Notice: â€Å"No one familiar with the mythologies of the primitive, ancient, and Oriental worlds can turn to the Bible without recognizing counterparts on every page, transformed, however, to render an argument contrary to the older faiths. In Eve’s scene at the tree, for example, nothing is said to indicate that the serpent who appeared and spoke to her was a deity in his own right, who had been revered in the Levant for at least seven thousand years before the composition of the Book of Genesis. There is in the Louvre a carved green steatite vase, inscribed c. 025 BC by King Gudaea of Lagash, dedicated to a late Sumerian manifestation of this consort of the goddess, under his title Ningizzida, ‘Lord of the Tree of Truth. ‘† p. 9. â€Å"The Serpent’s Bride. † Joseph Campbell. Occidental Mythology, The Masks of God. Arkana. New York. Viking Penguin Books. 1964, 1991 reprint The information in the Old T estament dates from about 1450 BC until 200 BC. This means that, contrary to the strongly held beliefs of most Christians, the creation story of Genesis is actually derived from the myths of ancient Sumerians. Therefore, the story is a myth. This means that those who against Darwin’s ideas on religious grounds based on their beliefs in the accuracy of the story in Genesis have unknowingly chosen to accept myth over facts. While they believe the myth to be factual, archeological evidence demonstrates otherwise. The scientific evidence leans heavily in support of Darwin’s ideas. While we may not fully understand some aspects behind the mechanism of evolution, we are continuously learning more about those mechanisms. (Dobzhansky, 1973) Dobzhansky states: Let me try to make crystal clear what is established beyond reasonable doubt, and what needs further study, about evolution. Evolution as a process that has always gone on in the history of the earth can be doubted only by those who are ignorant of the evidence or are resistant to evidence, owing to emotional blocks or to plain bigotry. By contrast, the mechanisms that bring evolution about certainly need study and clarification. There are no alternatives to evolution as history that can withstand critical examination. Yet we are constantly learning new and important facts about evolutionary mechanisms. Theodosius Dobzhansky, â€Å"Nothing in Biology Makes Sense Except in Light of Evolution†, American Biology Teacher vol. 35 (March 1973) reprinted in Evolution versus Creationism, J. Peter Zetterberg ed. , ORYX Press, Phoenix AZ 1983. Darwin (1859) believed that whales evolved from bears based on a scenario where selective pressures might cause this evolution, but he was criticized for this idea and removed the suggestion. Gould, 1995) Today, there is much more fossil evidence for the evolution of many species thus supporting the idea of evolution as a general biological principle, including the evolution of whales from lower animals. Evidence in support of evolution exists at many levels. There is paleontological evidence based on fossils, morphological evidence that relate the body morphology of higher animals to lower animals, evidence from molecular biology and from embryology. Added to this, the chronological picture that results is consistent with other lines of evidence. For example, the evidence for the evolution of whales from lower animals is convincing. Whales have been closely studied with respect to evolution. If evolution is valid, transitional stages from one level of evolution to another should exist. Although the fossil remains of whales spotty for a long time, recent fossil discoveries have more than adequately lent support to the concept of evolution for whales. Researchers state that independent lines of evidence from different disciplines confirm the pattern of evolution in whales. John Ray recognized that whales were mammals rather than fish in 1693 based on their similarity to terrestrial mammals. (Barnes, 1984) In 1883, Flower (see Barnes, 1984) found that whales had vestigial characteristics in common with terrestrial mammals just as humans have vestigial tails, the coccyx. Findings similar to these led to the concept of ‘ontogeny recapitulates phylogeny. ’ This concept is briefly explained in further detail below. Flower (1883) recognized that the whales have persistent rudimentary and vestigial features characteristic of terrestrial mammals, thus confirming that the direction of descent was from terrestrial to marine species. On the basis of morphology, Flower also linked whales with the ungulates; he seems to have been the first person to do so. Today, we know that whales have vestigial features in common with lower animals. For example, they have vestigial olfactory nerve, protruding hind limbs, pelvic fins and diaphragms. Like humans, during embryological development, whales develop features similar to lower animals and abandon them as development progresses. During their development, there is also evidence that whales have terrestrial ancestors. Some whales even develop hair while in the womb although they do not retain it. In 1985, Goodman et al. demonstrated that whales are more closely related to ungulates than to other animals. (Goodman, 1985; Miyamoto and Goodman, 1986) Some studies have identified genes, enzymes and other proteins that connect whales to extinct animals. (Irwin et al. 991; Irwin and Arnason, 1994; Milinkovitch, 1992; Graur and Higgins, 1994; Gatesy et al, 1996; Shimamura et al. , 1997) We have already noted above that the creation story in the Bible was taken from the text of an ancient culture that predates the Hebrew account. Rather than to openly acknowledge that the Bible’s story of creation is a mythical legend that explains evolution and the appearance of life on ea rth, some religious groups resort to far-fetched, fictitious, generally ridiculous concepts such as ‘creationism’, ‘creation science’ and ‘intelligent design’ to dismiss or explain away the science and replace it with fantasy. Embryology and developmental biology have a concept, ‘ontogeny recapitulates phylogeny’, that simplifies and briefly but succinctly expresses the concepts presented in the first chapter of Genesis. This is like explaining a complicated scientific concept, take conception and birth for example, to a little child by using a fairy tale rather than detailed research information. The fairy tale is not accurate, but the general information it communicates is true. With this single phrase, the first chapter of Genesis is summarized and explained. The phrase means that the embryological processes of development, ontogeny, depict and encapsulate the evolutionary history of the species, phylogeny. For example, during development of the human embryo, the fetus briefly has gills and a tail like its phylogenetic ancestors. In other words, during development, the developing embryo goes through some of the same stages that humans went through as the species developed from lower animals to humans. The concept makes perfect sense and explains many aspects of human development. Obviously, we cannot provide a comprehensive review of the evidence support evolution in a few pages, and we certainly cannot provide realistic evidence against it in light of all that exists to support it. While the evidence in support of the evolution of whales is plentiful, much evidence exists for evolution in general, including in humans. As has been reported here, the evidence is not just from scientific research, but also from archeology and history. That evidence shows, among other things, that the biblical story of creation in Genesis predates the Bible by hundreds of years. Despite all the evidence in support of evolution and against the idea of the biblical creation as being anything more than a myth, we can be certain that the argument in support of the biblical creation as being the real story and representing the real facts will not go away. Humans being what we are, we will always be faced with living with the Genesis myth as if it were fact, and coping with those who insist that the earth all the universe was created in six literal days. That concept certainly will never go away no matter what facts exist to disprove it.

Bad Survey Questions

EXAMPLES OF BAD QUESTIONS & SUGGESTIONS OF HOW TO FIX THEM! Double Barreled question: Please indicate how much you agree or disagree with each of the following statements about the childcare program. Incorrect: I feel welcomed by staff and other youth at the center Correction: I feel welcomed by staff at the center I feel welcomed by other youth at the center Biased/leading question Incorrect: Community organizing is hard. Do leadership trainings help you feel prepared for community organizing? Much more prepared Slightly more prepared Somewhat more prepared Not more preparedCorrect: The leadership trainings prepare me for community organizing. Strongly agree Double Negative Incorrect Does it seem possible or does it seem impossible to you that the Nazi extermination of the Jews never happened? (22%) Very possible Impossible Correct Do you doubt that the Holocaust actually happened or not? (9%) Very possible Impossible Possible Very impossible Possible Very impossible Agree Disagree Strongly disagree EVALUATING SURVEYS The following are additional examples of bad survey questions. As a group, apply our class discussion, evaluating each question and its response choices to identify one error in each question (ie. ouble barreled). Explain the problem that your group identifies in each question and propose an alternative that solves the problem. 1. More people have attended the movie, Gone with the Wind, than any other motion picture produced this century. Have you seen this movie? Yes No 2. In your opinion, how would you rate the speed and accuracy of your work? Excellent Good Fair Poor 3. Did you first hear about the bombing: ______ from a friend or relative ______ from your spouse ______ from a newspaper ______ at work ______ from the television or radio or other electronic media 4.How do you feel about the following statement? We should not reduce military spending. Strongly agree Agree Disagree Strongly disagree 5. People grow up in all different types of fam ilies. What type of family did you grow up in? ______Mom as single parent ______Dad as a single parent ______Both Mom and Dad 6. Where do you get most or all of your information about current events in the nation and the world? __radio __Newspapers __Magazines __internet 7. Higher than single-family density is acceptable in order to make housing affordable. Strongly Undecided Disagree Strongly Disagree Agree Agree 1 2 3 4 5CORRECTING SURVEYS The following are some suggestions of how to fix the problems of the survey questions found on the prior page. 1. Problem: Biased/Leading Solution: Have you seen the movie Gone with the Wind? Yes No 2. Problem: Double Barreled Solution: In your opinion, how would you rate the speed of your work? Excellent Good Fair Poor In your opinion, how would you rate the accuracy of your work? Excellent Good Fair Poor 3. Problem: Categories are not Mutually exclusive Solution: Did you first hear about the bombing: ____ from a friend ____ from a relative ___ _ from a media source 4.Problem: Double Negative Solution: How do you feel about the following statement? We should reduce military spending. Strongly agree Agree Disagree Strongly Disagree 5. Problem: Categories are not Exhaustive Solution: People grow up in all types of families. What type of family did you grow up in? ____two biological parents ____one biological parent & a step parent ____ mom as a single parent ____ dad as a single parent ____ with a relative other than my biological parents ____ in foster care ____ with adoptive parent(s) 6.Problem: Double Barreled Solution: Which media source do you rely on most to get information about current events involving the nation? ___radio ___ Newspapers __Magazines ___ Internet Which media source do you rely on most to get information about current events involving the world? ___radio ___ Newspapers __Magazines ___ Internet 7. Problem: Ambiguous Solution: In order to make housing affordable, is it acceptable to build housing whose b uildings hold more than one family? Strongly agree Agree Undecided Disagree Strongly Disagree

Adult Learners Postsecondary Academic Persistence Dissertation

Adult Learners Postsecondary Academic Persistence - Dissertation Example Actually, most community colleges rely on mature students with most of them learning part-time and staying outside the school. In the recent past, the mode of enrollment for most students has undergone a transformation with majority of them preferring to commute to and back from colleges (Bean and Metzner 485). The rise in the number of mature students was occasioned by massive dropouts of traditional students thus forcing college administrators to come up with a model that could take in mature students to replace those who were dropping out. Despite the high number of adult students joining colleges, trends also show that such students have an unlikely possibility of completing their studies. Majority of the non-traditional students live outside the school setting and this aspect alone sets them apart from the traditional students. Majority of these non-traditional are also part-timers since most of them are either working or taking care of families. Due to lack of adequate contact with the student’s faculty, non-traditional students are less likely to complete their study program (Bean and Metzner 485). Q.2 Commuter can come from any part of the country. Given the nature of these students, they are unable to reside within the schools and they therefore have to commute hence earning themselves the name commuter students. On their part, traditional students are classified as 18-24 years and they attend college full-time. By virtue of being mature, the commuter students have already developed self-control and values that usually come with being mature. This makes them less susceptible to socialization unlike their traditional counterparts (Garni 465). In many instances, traditional students are registered for degree programs than any other. While on the other hand, commuter students might be registered for a degree or any other programs. The commuter student is not mostly likely to be affected by what the college has to offer and most of them do not conti nue with the program after they are assured that they have attained the qualification that they were looking for. Most commuter students are usually in a certain location for some time and if for example one is transferred from their place of work, then they have to discontinue the program. This means that commuter students are less likely to continue with their academic programs as compared to their residence counterparts (Garni 466). Q.3 It is evident; there is a clear connection between a student’s high school GPA and their college performance. Students who demonstrate a low academic performance are most likely to drop out compared to their counterparts who perform well. Research has also shown that a student’s college GPA is strongly linked to their past academic performances. Although a student’s high school grades may not be directly linked to the rate of dropout, there is a clear link between high school grades and college grades and low grades are a key factor in the rate of drop-outs (Bontrager 9). Ideally, the academic outcome has a direct link with the psychological outcome. Students who attain good grades are more likely to stay in school compared to those who perform poorly. Research shows that most commuter students had a low GPA in high school and this translates to low GPA in their college education. In reality, even if a student has a low academic outcome but get adequate psychological support,

Strategic Management - Strategic Audit Format Essay

Strategic Management - Strategic Audit Format - Essay Example In other words, the computing sector is infinitely dependent on industrial growth. However, a contradiction arises as resources are not infinite in nature. There are also several sustainability issues which have always been a challenge for this industry. 3. Scarcity of fossil fuel: The computer industry relies on low-priced energy. Cheap energy sources generally come from fossil fuel. The scarcity of fossil fuel has always been a concern for the industry as availability of energy sources to power devices at reasonable price is vital. 4. Environmental Issues: The computer industry is one of the main reasons for the growing pollution in the developing nations. A huge amount of waste is generated and there have been challenges associated with the recycling and reduction of waste. Manufacturing of computers also involve the use of hazardous chemicals like lead and mercury which pollutes the environment. 1. Maintenance of confidentiality – Protection of privacy of the users has to be ensured by the computer industry. This is a major challenge for this industry as, with the increased linkages between network connections, it is important that computers are supported with tools that help to maintain privacy of the users. There are threats that arise from virus and malevolent software. Increased use of the internet has aggravated such threats. The industry needs to give protection against malwares which pose a threat during the use of confidential information over the net. 2. Technically support the customers – The computer industry not only needs to concentrate on manufacturing of computer hardware but also needs to ensure that all further technical difficulties faced by the customers are looked into and proper solutions are provided. There has to be a continuous link with the customers so that support and assistance can be provided at odd hours. This is a major challenge for the computer industry. 3.

DNA Sequencing Essay Example | Topics and Well Written Essays - 4000 words

DNA Sequencing - Essay Example The Sanger methods are able to sequence best from 30-350 nucleotides and therefore genomic sequencing strategies have been developed to sequence longer DNA of interest such as the gene of interest in this plant. In the shotgun sequencing strategies, DNA often of large size is shredded into smaller fragments that can then be sequenced individually. Shredding of the DNA is done by restriction enzymes or mechanically by shearing the DNA. The sequences of these fragments are then reassembled into their original order based on overlaps. Alignment of the sequences is done by a computer program to yield the complete sequence. In Whole-genome shortgun, the DNA is obtained without prior physical map knowledge and indiscriminately sheared into fragments of 100kb which are then cloned into plasmids and transformed. The DNA inserts obtained from the plasmids are sequenced individually and consequently assembled into a long contiguous sequence. The strategy has limits due to gaps which arise duri ng assembly due to the repeats in the sequences. Another strategy is primer walking which tends to deal with whole shortgun sequencing challenges in the assembly of â€Å"gaps†. Clones carrying inserts for sequences for both sides of the gap are identified and the DNA is sequenced normally. Resultant sequence is used to design a primer downstream from the former primer position. Pairwise-end sequencing is another strategy for genome sequencing which is performed on both sides of DNA of interest as opposed to one in whole-genome shortgun.... Usually alignment of the sequences is done by a computer program to yield the complete sequence. In Whole-genome shortgun, the DNA is obtained without prior physical map knowledge and indiscriminately sheared into fragments of 100kb which are then cloned into plasmids and transformed. The DNA inserts obtained from the plasmids are sequenced individually and consequently assembled into a long contiguous sequence. The strategy has limits due to gaps which arise during assembly due to the repeats in the sequences. Another strategy is primer walking which tends to deal with whole shortgun sequencing challenges in assembly of â€Å"gaps†. . Clones carrying inserts for sequences for both sides of the gap are identified and the DNA is sequenced normally. Resultant sequence is used to design a primer downstream from the former primer position. These steps are repeated over and over until the complete sequence of the insert is elucidated. Pairwise-end sequencing (double-barrel shortgun ) is another strategy for genome sequencing which is performed on both sides of DNA of interest as opposed to one in whole-genome shortgun. It reduces â€Å"gaps† thereby minimizing assembly errors which are common in whole-gun sequencing. However it poses a huge computational challenge during assembly. DNA is shredded into 150mb fragments and inserted into BACs in hierarchical shortgun sequencing strategy. Inserts are mapped into a physical map and organized by known location â€Å"Golden Tiling Path†. Inserts are fragmented further and cloned into plasmid where they are again recovered and sequenced according to â€Å"the Golden Tiling Path†. This strategy is applied for long pieces of DNA such as whole genome or chromosome and in